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Fig. 2 Early expression of pitx2 transcripts during zebrafish development. (A) Northern blot of total RNA prepared at the indicated stages. The blot was probed with an antisense RNA probe corresponding to the whole zpitx2c cDNA. (B) RT-PCR analysis showing that pitx2c transcripts start to accumulate at the onset of zygotic transcription. (C–G) Whole-mount in situ hybridization with a probe recognizing all the pitx2 isoforms showing expression of pitx2 first in the presumptive mesendoderm and then in prechordal mesoderm. (C) Sphere, (D) 30% epiboly, (E) germ ring, (F) shield, (G) 75% epiboly.
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Reprinted from Developmental Biology, 229(2), Faucourt, M., Houliston, E., Besnardeau, L., Kimelman, D., and Lepage, T., The pitx2 homeobox protein is required early for endoderm formation and nodal signaling, 287-306, Copyright (2001) with permission from Elsevier. Full text @ Dev. Biol.