ZFIN Image: Chiang et al., 2001, Fig. 11

Image

Figure Caption

Fig. 11 Transactivation domain analysis of Sox9a and Sox9b. (A) Structure of expression plasmids. The Gal4 DNA-binding domain (DBD) was linked to different lengths of the C-terminal domain or full-length Sox9 protein. The numbers above each box represent residue numbers. The reporter contains the chloramphenicol acetyltransferase (CAT) gene driven by a minimal TATA box and 5 copies of the Gal4 recognition sequence. (B) CAT activity of the reporter pG5E1bCAT cotransfected with different effector plasmids after normalization. (C) Western blot of 20 μg protein from each cell lysate after transfection probed with anti-Gal4 antibody. Effector proteins could be detected in all transfections.

Acknowledgments

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Reprinted from Developmental Biology, 229, Chiang, E., Pai, C.-I., Wyatt, M., Yan, Y-L., Postlethwait, J., and Chung, B.-C., Two sox9 genes on duplicated zebrafish chromosomes: expression of similar transcription activators in distinct sites, 149-163, Copyright (2001) with permission from Elsevier. Full text @ Dev. Biol.