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Fig. 4 Insulin and Pdx-1 expression after shh overexpression in WT embryos. Left: mRNA control-injected embryos. Right; shh mRNA-injected embryos. After mRNA injection at the two- to four-cell stage, embryos developed for 24 or 48 hpf and were subjected to whole-mount in situ analysis using the insulin or Pdx-1 probe. (A, C, E) Control GFP mRNA-injected embryos hybridized at 24 hpf with the insulin probe. Positive cells are centered ventral to somite 4 (4). (B, D, F) Shh mRNA-injected embryos hybridized with the insulin probe. Note the anterior extension of labeled cells. (G) Control β-gal mRNA-injected embryo hybridized with the Pdx-1 probe. (H) Shh mRNA-injected embryo hybridized with the Pdx-1 probe. (A, B, E, F) Ventral views. (C, D) Lateral views. (G, H) Dorsal views.
Reprinted from Developmental Biology, 244(1), diIorio, P.J., Moss, J.B., Sbrogna, J.L., Karlstrom, R.O., and Moss, L.G., Sonic hedgehog is required early in pancreatic islet development, 75-84, Copyright (2002) with permission from Elsevier. Full text @ Dev. Biol.