ZFIN Image: Rastegar et al., 2002, Fig. 5

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Figure Caption

Fig. 5 The Shh signalling pathway does not control the activity of the –1.1netrin1:gfp/FPE transgene. (A, B) Expression of endogenous netrin1 mRNA in an uninjected control (A) and a shh mRNA-injected embryo (B). Ectopic expression of shh causes expansion of endogenous netrin1 expression in the neural tube (arrowheads). (C, D) Expression of gfp mRNA in control uninjected (C) and shh-injected transgenic embryo (D). In contrast to the endogenous netrin1 gene, the –1.1netrin1:gfp/FPE transgene does not respond to ectopic shh. (E, F) Transgene expression in a wildtype control embryo (CO, E) and Smu^b641 mutant background (F). –1.1netrin1:gfp/FPE expression is not significantly different in the floor plate of wildtype and smu mutant embryos. Orientation of embryos: anterior left, dorsal up. Abbreviations: f, floor plate; h, hypochord; CO, control. Scale bar represents 100 μm.

Acknowledgments

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Reprinted from Developmental Biology, 252(1), Rastegar, S., Albert, S., Le Roux, I., Fischer, N., Blader, P., Müller, F., and Strähle, U., A floor plate enhancer of the zebrafish netrin1 gene requires Cyclops (Nodal) signalling and the winged helix transcription factor FoxA2, 1-14, Copyright (2002) with permission from Elsevier. Full text @ Dev. Biol.