ZFIN Image: Rastegar et al., 2002, Fig. 1

Image

Figure Caption

Fig. 1 A PAC clone comprising the netrin1 locus drives expression of reporter gene in the ventral neural tube. (A) Map of the netrin1 PAC clone. Blow-up shows the promoter region and the position of the green fluorescent protein (gfp) tag brought under control of the netrin1 regulatory region by insertion into the first coding exon. (B) Embryo (24 hpf) expressing the gfp-tagged netrin1 PAC clone. Mosaic expression of GFP can be detected in cells of the floor plate (arrows) and the hypochord (arrow heads). (C) Embryo (24 hpf) hybridised to the netrin1 antisense probe. (B, C) Lateral views, anterior left and dorsal up. The scale bar represents 100 μm in (B) and (C). Abbreviations: N, NotI; B, BamHI; Bs, BssHII; Kb, kilobase pairs; ATG, transcription start; f, floor plate; h, hypochord.

Acknowledgments

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Reprinted from Developmental Biology, 252(1), Rastegar, S., Albert, S., Le Roux, I., Fischer, N., Blader, P., Müller, F., and Strähle, U., A floor plate enhancer of the zebrafish netrin1 gene requires Cyclops (Nodal) signalling and the winged helix transcription factor FoxA2, 1-14, Copyright (2002) with permission from Elsevier. Full text @ Dev. Biol.