ZFIN Image: Wu et al., 2002, Fig. 5

Image

Figure Caption

Fig. 5 Detection of rPTPα in the zebrafish egg: Co-immunoprecipitation with Fyn. The left panel demonstrates a phosphatase assay of immunoprecipitates prepared from 1000 zebrafish zygotes (30 min postfertilization) using anti-rPTPα-35 at 1:400 dilution (●) or control rabbit serum at the same dilution (○). The anti-rPTPα-35 immunoprecipitates contained PTPase activity evident as the removal of almost all ³²PO₄ from the peptide which eluted in fractions 10 and 11. The right panel demonstrates that the anti-rPTPα-35 antibody detects a 130-kDa protein in Western blot analysis of Fyn immunoprecipitates prepared from the membrane fraction of 1500 eggs (B). This band was not seen in blots probed with control rabbit IgG (A).

Acknowledgments

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Reprinted from Developmental Biology, 247(2), Wu, W. and Kinsey, W.H., Role of PTPase(s) in regulating Fyn kinase at fertilization of the zebrafish egg, 286-94, Copyright (2002) with permission from Elsevier. Full text @ Dev. Biol.