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Fig. 5 Ectopic shh expands lim3 and nk2.2 expression and induces PRL and SL cell populations. (A, B) lim3 expression in GFP mRNA-injected (A) and shh mRNA-injected (B) embryos. shh mRNA injections lead to posterior expansion of the lim3 expression domain (arrow in B). (C, D) nk2.2 expression in GFP mRNA-injected (C) and shh mRNA-injected (D) embryos. nk2.2 expression is expanded within the developing placode (compare arrows in C and D). (E–H) PRL cell populations in GFP (E, G) and shh (F, H) mRNA-injected embryos. The number of PRL-producing cells is consistently expanded in the anterior region of the adenohypophysis. (I) SL-producing cells are present throughout the adenohypophysis (bracket) in control (GFP) RNA-injected embryos. (J) Ectopic SL cells (arrows) are induced after shh mRNA injection. All panels show 30-h embryos that were injected with 100 pg of the indicated mRNA at the two- to four-cell stage. (A, B, E, F, I, J) Ventral views, anterior up. (C, D, G, H) Lateral views, eyes removed, anterior to the left. Brackets show anterior/posterior extent of adenohypophysis. di, diencephalon.
Reprinted from Developmental Biology, 254(1), Sbrogna, J.L., Barresi, M.J., and Karlstrom, R.O., Multiple roles for Hedgehog signaling in zebrafish pituitary development, 19-35, Copyright (2003) with permission from Elsevier. Full text @ Dev. Biol.