Fig. S4

Fig. S4 Smoothened Function in Adaxial and Lateral Floor Plate Cells Does Not Appear to Be Required for the Induction of Dorsal Pancreatic β-Cells
(A) Schematic diagram of the cell transplantation protocol. Wildtype Tg(ins:GFP) donor cells were transplanted into wildtype or smo mutant Tg(ins:GFP) hosts. (B-G) Transverse confocal images of wildtype (B and E) and smo mutant (C, D, F and G) hosts stained for GFP (green) and β-catenin (white) at 18 hpf. Wildtype donor cells are labeled with rhodamine dextran (red). (B and E) Regardless of the position of the wildtype donor cells, Tg(ins:GFP) expression is unaffected in wildtype hosts. (C, D, F and G) 13 Tg(ins:GFP) expression is not rescued in smo mutant hosts when wildtype donor cells differentiate into adaxial (C and F; arrowhead) or lateral floor plate (D and G; arrowhead) cells in the pancreatic region, i.e. between the 1^st and 4^th somites.