IMAGE

Fig. 6

ID
ZDB-IMAGE-080411-26
Source
Figures for Pagnon-Minot et al., 2008
Image
Figure Caption

Fig. 6 The lack of collagen XV disrupts notochordal basement membrane and prevents normal muscle striation. Transmission electron micrographs of the notochord (A, B) and of the myotome of 48 hpf control (A, C, E) and morphant (B, D, F–I) zebrafish. (A) In wild type and in control embryos, basement membrane surrounding the notochord (no) is constituted by three different layers: a true basement membrane (bm, arrow), a dense fibrous layer (dl) that surrounds the notochord (black bar), and an outermost fibrous layer (ol) parallel to the notochord (white bar). (B) In Col15a1-MO embryos, the basement membrane is highly disorganized. In the dense fibrous layer (dl), collagen fibers are not properly arranged. The outermost fibrous layer (ol) is less dense and more diffuse compared to control. (C, E) In 48 hpf control embryos, myofibrils (my) are completely developed and the cytoplasm is overgrown with myofibrils. As observed in the longitudinal section (E) muscle striation is complete. (D, F–H) In Col15a1-MO embryos, the number of myofibrils (my) is low. Myofibrils are preferentially located in the cortical area of the cells (C) and often present an orthogonal arrangement (G). (F–H) Muscle striation (s) is more or less visible depending on the severity of the morphotype. (I) Multivesicular vesicles which represent a sign of muscle degeneration are observed. Bar: 2 μm.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 316(1), Pagnon-Minot, A., Malbouyres, M., Haftek-Terreau, Z., Kim, H.R., Sasaki, T., Thisse, C., Thisse, B., Ingham, P.W., Ruggiero, F., and Le Guellec, D., Collagen XV, a novel factor in zebrafish notochord differentiation and muscle development, 21-35, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.