ZFIN Image: Xing et al., 2008, Fig. 4

Image

Figure Caption

Fig. 4 The Role of Helix C in β-Catenin Protein-Protein Interactions

(A) Potential interactions between β-catenin helix C and ICAT. β-catenin-R1C (βcat-R1C, cyan) is superimposed onto β-catenin/ICAT (βcat/ICAT, yellow and magenta), based on the Cα's of β-catenin armadillo repeats 10?12 (residues 563?663).

(B) Helix C is unlikely to affect the interaction between the phophorylated E-cadherin and β-catenin armadillo repeats. β-catenin-R1C (βcat-R1C, cyane) is superimposed with β-catenin/phospho-Ecadherin (βcat/pEcad, yellow and red, PDB code: 1I7W) based on all twelve armadillo repeats.

(C) The helix C is required for β-catenin to interact with Chibby. Purified MBP-Chibby protein was immobilized to amylose beads; purified β-catenin and β-catenin fragments were tested for their binding to immobilized MBP-Chibby. The total input and bound β-catenin and β-catenin fragments were visualized by Western blot.

Acknowledgments

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Reprinted from Structure (London, England : 1993), 16(3), Xing, Y., Takemaru, K., Liu, J., Berndt, J.D., Zheng, J.J., Moon, R.T., and Xu, W., Crystal Structure of a Full-Length beta-Catenin, 478-487, Copyright (2008) with permission from Elsevier. Full text @ Structure