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Fig. 5 RT-PCR analysis of the NLR gene family in intestine (1 and 2), liver (3 and 4) and spleen (5 and 6) of two individual naïve zebrafish. ARP expression was amplified to verify cDNA synthesis. Negative controls were performed using templates from cDNA synthesis reactions without reverse transcriptase (7). Genomic DNA (8) was amplified to verify primer efficiency and to show the difference in size of genomic amplicons compared to cDNA amplicons.
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Full text @ BMC Evol. Biol.