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Fig. 9 Foxd3 can physically interact with predicted forkhead binding sites in the mitfa promoter. Electrophoretic mobility shift assay (EMSA) using synthetic Foxd3 protein, radiolabeled conserved region probes spanning the predicted forkhead binding sites (Site 1 and Site 2), and unlabeled wild-type or mutated site 1 or site 2 or unrelated (OCTA) oligonucleotide competitors. Arrows denote specific bands observed with Foxd3 protein. wt—wild-type site 1 or site 2 competitors; Mut—mutated site 1 or site 2 competitors; NS—nonspecific (OCTA) competitor; FP—free probe (arrowhead).
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Reprinted from Developmental Biology, 313(2), Ignatius, M.S., Moose, H.E., El-Hodiri, H.M., and Henion, P.D., colgate/hdac1 repression of foxd3 expression is required to permit mitfa-dependent melanogenesis, 568-583, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.