Fig. 1 Expression patterns of robo4 in development. A montage of robo4 whole-mount in situ staining is shown, with 16 (A), 19 (B), 20 (C), 23 (D), 24 (E), and 20 (F) som. (G) High power of F.(H) High power of I.(I) A 22-som telencephalon (t), hindbrain (hb), and neural tube (nt). J is 28-29 hpf. Arrowhead in G indicates expression in angioblasts; asterisk in H shows ISV expression. robo4 (K), G-RCFP (L), DAPI (M), and merge (N) show two-color staining of frozen 20-μm anterior trunk sections of vegfr2-G-RCFP 25-hpf embryos. The arrowhead and asterisk in K-N indicate ISV and axial vessels, respectively. Images were taken with a x20 (A-E), x10 (F, I, and J), x40 (G and H), and x20 (K-N) lens with x2.5 zoom.
Image
Figure Caption
Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and
ZFIN has permission only to display this image to its users.
Additional permissions should be obtained from the applicable author or publisher of the image.
Full text @ Proc. Natl. Acad. Sci. USA