ZFIN Image: Raschperger et al., 2008, Fig. S1

Image

Figure Caption

Fig. S1 Characterization of the anti-CAR SIV antibodies against human and zebrafish CAR. Immunofluorescent and immunoblot analysis of human (hCAR) and zebrafish (zCAR) CAR transfected QBI-293A cells (293). The anti-CAR SIV antibodies detected exogenously expressed hCAR and zebrafish zCAR at cell–cell contacts as well as at the cell-surface of transfected 293 cells (A and B, respectively). Immunoreactivity of the antibody against endogenously expressed hCAR in 293 cells is indicated with arrowheads (A and B). (C) The anti-CAR SIV antibodies react with an approximately 47 kDa protein band in cell lysates from exogenously expressed hCAR and zCAR in transfected 293 cells (lanes 2 and 3, respectively). Endogenously expressed hCAR was used as control for the molecular size (lane 1). The position of the molecular weight marker (kDa) is indicated to the left.

Acknowledgments

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Reprinted from Developmental Biology, 313(1), Raschperger, E., Neve, E.P., Wernerson, A., Hultenby, K., Pettersson, R.F., and Majumdar, A., The coxsackie and adenovirus receptor (CAR) is required for renal epithelial differentiation within the zebrafish pronephros, 455-464, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.