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Fig. 5 CAR morphants display loss of endogenous alkaline phosphatase activity in pronephric tubules. Transversal sections through pronephric tubules (arrowheads) of control and CAR morphants at 96 hpf were incubated in NBT/BCIP. Reduction of endogenous alkaline phosphatase activity in apical duct cells was observed in CAR morphants (B) as compared to controls (A).
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Reprinted from Developmental Biology, 313(1), Raschperger, E., Neve, E.P., Wernerson, A., Hultenby, K., Pettersson, R.F., and Majumdar, A., The coxsackie and adenovirus receptor (CAR) is required for renal epithelial differentiation within the zebrafish pronephros, 455-464, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.