Fig. 2

Fig. 2 CAR protein expression in zebrafish pronephros. Immunohistochemical co-stainings of CAR with the tight junctional protein ZO-1 were performed on transversal sections of the pronephros from a 96 hpf zebrafish larvae. (A) CAR and ZO-1 were detected in parietal epithelium (pe) of the Bowman's capsule that surrounds the pronephric glomerulus (pg) and in the gut (g). Podocyte and endothelial expression of ZO-1 was also detected in the pronephric glomerulus. (B) In the pronephric tubules (pt), CAR and ZO-1 co-localize to epithelial cell–cell contacts close to the lumen (asterisks in panels B and C), and this apical localization was verified by co-staining of CAR and the basolateral marker Na⁺/K⁺-ATPase alpha1 subunit (C). The notochord (n) is indicated in panel A for orientation and DAPI was used to visualize the nuclei (blue). Scale bar, 50 μm.