Fig. 2

Fig. 2 YSL nuclei are present but disordered in mtx2 morphants. (A–H) Fluorescence microscope images of SYTOX Green injected embryos. Wild-type (A–C) and mtx2 MO (D–F) injected embryos (two- to four-cell stage), injected at 1000-cell stage with SYTOX Green. (A, D) Arrowheads indicate YSN at the blastoderm margin. mtx2 MO injected embryos have a similar collection of YSN at the blastoderm margin (arrowheads in panel D) but show an abnormal distribution of YSN in the inner YSL (I-YSL), which persists (E) until just before yolk cell lysis (F) when the YSN disintegrate. High magnification, merged bright field/fluorescence imaging (G, H) shows the majority of I-YSL nuclei are evenly spaced in wild-type embryos (G) but randomly distributed in mtx2 MO injected embryos (H). Scale bar = 100 μm in panel F (applies to panels A–F), panels G and H. (I) YSN spatial distribution analysis. At distances beyond the diameter of each YSL nucleus (shaded red zone), wild-type YSN are dispersed with maximal deflection from complete spatial randomness (CSR) at r = 22 μm (asterisk indicates significance p value < 0.001). However, in mtx2 morphants, the nuclei are randomly distributed. K-functions are means for wild-type (n = 10) and mtx2 MO (n = 6) embryos, standardized on the 99% confidence interval (CI).