Fig. 3

Fig. 3 dlx2a is necessary for survival of a subset of neural crest cells migrating to the pharyngeal arches. Crestin expression at 13 hpf in (A) Control (un-injected) embryo compared with, (B) an embryo injected with dlx2aMO, 0.25 mM. (B, inset) crestin expression at 15 hpf in dlx2aMO-injected embryo (0.25 mM). (C, D) Acridine orange staining of (C) control un-injected and (D) dlx2aMO-injected (0.5 mM) embryos at 14 hpf. (E, F) TUNEL-labeled transverse sections of a control (un-injected) 16 hpf embryo and an embryo injected with 0.25 mM dlx2aMO, (G, H) TUNEL labeling (black) and crestin (red) whole-mount in situ hybridization at 19 hpf. (G) Control (un-injected) embryo compared with (H) dlx2aMO injected embryo, 0.25 mM. Arrowheads in panels A and B indicate reductions of rostral crestin expression. Arrowheads in panels C, D, H indicate sites of cell death. (G, H) crestin labeled neural crest streams are labeled with Roman numerals. Anterior is to the left. cns, central nervous system; e, eye; m, mesoderm; nc, neural crest; ov otic vesicle; y, yolk. Scale bar: (A, G) 100 μm, (E) 50 μm.