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Fig. S3 Heart muscle ultrastructure is unaffected by the sloθ45 mutation. Transverse TEM sections through the heart tube at 48 hpf reveal a thin myofibrillar band in the heart wall in both siblings (A) and slou45 mutants (C). Z-disc to Z-disc distances show no obvious differences between the mutant and sibling (arrowheads, A and C). Longitudinal sections reveal some myofibrils in cross section (boxes, B and D) and in these cases it is clear that the myofibrils contain both thick and thin filaments. RBC, red blood cell. Scale bars: 500 nm in A,C; 200 nm in B,D.
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