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Fig. S11 Comparison and rescue of the EVL lamellipodia phenotype. Animal pole view confocal z-projections of Alexa488-Phalloidin stained EVL cells at 90% epiboly, depicted using the depth coding method. (A) WT; (B) MZspghi349; (C) MZspgm793. Extensive lamellipodia formation is observed in both alleles. (D–F) Depicts a rescue experiment showing (D) WT, (E) MZspghi349 non-injected, and (F) MZspghi349 injected with 50 pg pou5f1 mRNA. Genetically MZspghi349 mutant embryos are completely rescued by pou5f1 mRNA injection with respect to their EVL lamellipodia phenotype. Scale bar: 20 μm.
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Reprinted from Developmental Biology, 315(1), Lachnit, M., Kur, E., and Driever, W., Alterations of the cytoskeleton in all three embryonic lineages contribute to the epiboly defect of Pou5f1/Oct4 deficient MZspg zebrafish embryos, 1-17, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.