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Fig. S7 Excessive formation of cortical layer spherical structures. (A, B) Z-projection of anti-β-Tubulin immunofluorescence stain (Alexa488 conjugated α mouse-Ab) of MZspgm793 embryos. (A′, B′) Confocal slices depicting sphere localization within the cortical layer. (A) Mild MZspgm793 phenotype. (B) Severe MZspgm793 phenotype. Scale bar: 100 μm.
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Reprinted from Developmental Biology, 315(1), Lachnit, M., Kur, E., and Driever, W., Alterations of the cytoskeleton in all three embryonic lineages contribute to the epiboly defect of Pou5f1/Oct4 deficient MZspg zebrafish embryos, 1-17, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.