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Fig. S3 Maternal Pou5f1 impact on the DEL and EVL phenotype. (A–C) Z-projection of confocal image stacks of 85% epiboly embryos stained with Sytox green to visualize nuclei. (A) WT; (B) Mspgm793; (C) MZspgm793. A delay of EVL and DEL is detectable based on altered distribution of nuclei. (D–F) Higher magnification of the EVL margin of confocal z-projections of Alexa488-Phallodin stained embryos. (D) WT; (E) Mspgm793; (F) MZ spgm793. Note delay in change of marginal EVL cell shape and reduced stain intensity of the punctate F-Actin band in the YSL. yc: yolk cell. Scale bar: 100 μm.
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Reprinted from Developmental Biology, 315(1), Lachnit, M., Kur, E., and Driever, W., Alterations of the cytoskeleton in all three embryonic lineages contribute to the epiboly defect of Pou5f1/Oct4 deficient MZspg zebrafish embryos, 1-17, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.