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Fig. S1 Transplantation of Prethalamic Precursors in Ectopic Environment (A–C) At 22 hpf (dorsal view, rostral to the left), ectopic expression of dlx2a inside part of the clone cells expressing the prethalamic marker form an ectopic epithelial structure (arrow in [A]). (D–F) At the 10-somite stage (lateral view, rostral to the left), ectopic expression of the prethalamic marker lhx5 could be found in a subset of the transplanted cells, but no ectopic expression of the telencephalon marker emx3 in that basally located clone was found. (G–I) At 24 h, cyclopamine-treated from bud to 24 h, successful treatment with cyclopamine leads to the development of big and almost fused eyes ([G], asterisk); no hypothalamus is detectable. (H) Endogenous expression of lhx5 (arrow) is still detectable in the prethalamus of cyclopamine-treated embryos. (I) Dorsal view, rostral to the left: ectopic expression of lhx5 by the transplantation of anterior diencephalic cells after cyclopamine treatment (arrow) is shown.