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Fig. 7 Transplantation of Prethalamic Precursors (A) Schematic overview of the transplantation experiment putting prethalamic precursors in ectopic regions of the neural plate. (B–D and H–J) shows dlx2a (blue), tbr1 (red), and transplanted cells in green; (E–G and K–P) shows lhx5 (blue), irx1b (red), and transplanted cells in green. (B–G) Transplanted cells located in the hindbrain show ectopic expression of the prethalamus markers dlx2a (B–D) or lhx5 (E–G); no expression of the telencephalon marker tbr1 (see inset in [B]) could be found in the clone. Cells do not exhibit expression of irx1b (arrow in [F]) in the transplanted cells. (H–P) After transplantation into the forebrain, ectopic cell–non-autonomous expression of dlx2a (arrow in [J]) could be seen (probably resulting in the split of the telencephalon, arrow in [I]) as well as of lhx5 in the thalamus (arrow in [M]). Cells did not switch on the expression of thalamic irx1b (arrow in [O]) in the thalamus.