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Fig. 8 Identification of an endothelial enhancer in the fli1a first intron. A: Schematic description of polymerase chain reaction and BP cloning approach to generate overlapping 1-kb fragments for screening enhancer activity. B: Description of LR multisite cloning to generate injection constructs. C-E: Transmitted light images of embryos immunostained with an antibody against Egfp; embryos were co-injected with 25 pg of indicated Tol2 construct and 25 pg of Tol2 transposase mRNA.C: Expression of Egfp in blood vessels of the head and trunk in an embryo injected with pTol-fli1a-0.9/+5.9:egfp. D: Ectopic Egfp expression in the head and trunk in an embryo injected with pTol-fli1a+1.2/+2.2:egfp. E: Egfp expression in head vessels (arrows) and trunk vessels (arrowhead) in an embryo injected with pTol-flia+2.2/+3.2:egfp.