Fig. 8

Fig. 8 Structural and functional conservation of CREST3 during evolution. (A) Sequence comparisons between CREST3s. No similar sequences to CREST3 were found in the fugu genome. Only the most conserved regions among species are shown. The entire nucleotide sequences of zCREST3, cCREST3, mCREST3, and hCREST3 were deposited in DDBJ under accession numbers AB0158305, AB167734, AB158308, and AB158311, respectively. The nucleotides conserved in more than three of all species are shaded by the red color. The bracket indicates the conserved region between CREST3s and C3Rs (Fig. 9D). (B and C) Lateral views of 36-hpf embryos injected with the zCREST3-isl1:GFP plasmid and exhibiting expression of GFP in trigeminal ganglion neurons (B) and Rohon-Beard neurons (C). (D and E) Lateral views of 24-hpf Tg(zCREST2-hsp70:GFP)^rw011 embryos injected with the zCREST3-isl1:mRFP1 plasmid and exhibiting mRFP1 expression in trigeminal ganglion neurons (D) and Rohon-Beard neurons (E). Note that there are some mRFP1 single-positive neurons in both trigeminal ganglion neurons and Rohon-Beard neurons (arrowheads).