ZFIN Image: Machingo et al., 2006, Fig. 7

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Figure Caption

Fig. 7 Laminin is improperly galactosylated in β4GalT1 morphant embryos. (A) Control and β4GalT1MO embryos were lysed, and laminin was immunoprecipitated with a-laminin antibodies. The immunoprecipitate was resolved by SDS-PAGE and probed for RCA-I reactivity. Control embryos have strong RCA reactivity at 400 kDa reflecting the α chain of laminin, as well as at 200 kDa, representing the β and γ chains. β4GalT1MO embryos retain only weak RCA reactivity to the 400-kDa protein and almost no RCA reactivity at 200 kDa. (B) To confirm that β4GalT1MO embryos still express laminin, control-injected and β4GalT1-morpholino-injected embryos were stained for laminin. Control embryos (6 somites) have laminin expression along the notochord as well as along the lateral mesoderm of the head. β4GalT1MO embryos (12 somites) retain laminin expression along the notochord, although the domain is wider.

Acknowledgments

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Reprinted from Developmental Biology, 297(2), Machingo, Q.J., Fritz, A., and Shur, B.D., A beta1,4-galactosyltransferase is required for convergent extension movements in zebrafish, 471-482, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.