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Fig. 4 Rescue of the bty phenotype by co-injection of an antisense MO and a synthetic bty mRNA with an artificial 5′-UTR. Embryos were stained with o-dianisidine at 32 hpf to detect the hemoglobin-expressing red blood cells. (A) Wild-type embryo, uninjected. (B) Embryo injected with Zebb302a antisense MO, 5 ng. (C) Embryo co-injected with Zebb302a MO (5 ng) and synthetic bty mRNA (10 pg). (D) Embryo co-injected with Zebb302a MO (5 ng) and synthetic bty mRNA (100 pg).
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Reprinted from Developmental Biology, 283(1), Yergeau, D.A., Cornell, C.N., Parker, S.K., Zhou, Y., and Detrich, H.W. 3rd., bloodthirsty, an RBCC/TRIM gene required for erythropoiesis in zebrafish, 97-112, Copyright (2005) with permission from Elsevier. Full text @ Dev. Biol.