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Fig. 4 Embryonic expression of Zangptl6. Whole-mount in situ hybridization was done with a Zangptl6 probe at 14hpf (A), 18hpf (B) and 24hpf (C–E) and with a shh probe at 24hpf (F,G). In an 14hpf embryo (A), Zangptl6 expression was seen in almost entire length of notochord. In a 18hpf (B) and 24hpf (C) embryos, Zangptl6 expression was restricted to the caudal end of notochord. Higher magnification of a 24hpf embryo is shown in (D). In sagittal sections of a 24hpf embryo (E), Zangptl6 expression was detected in the notochord ventral to the spinal cord. High magnification lateral view (F) and a sagittal section (G) of a 24hpf embryo stained with shh as a marker of notochord are shown. Closed arrowheads, notochord; sc, spinal cord. Bars indicate 50 μm.
Reprinted from Gene expression patterns : GEP, 5(5), Kubota, Y., Oike, Y., Satoh, S., Tabata, Y., Niikura, Y., Morisada, T., Akao, M., Urano, T., Ito, Y., Miyamoto, T., Watanabe, S., and Suda, T., Isolation and expression patterns of genes for three angiopoietin-like proteins, Angptl1, 2 and 6 in zebrafish, 679-685, Copyright (2005) with permission from Elsevier. Full text @ Gene Expr. Patterns