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Figure Caption
Fig. 5 Morphology and distribution of cell nuclei in normal and blocked embryos. Embryos were injected with c-Fyn (A–D) or FynK299M mRNA (E–H) as in Fig. 4. When the control embryos reached 50% epiboly, the embryos were fixed in 2% glutaraldehyde, permeabilized with 0.1% TX 100, and stained with DAPI. The embryos were then examined with bright field (A, C, E, G), or with fluorescence microscopy to localize the DAPI-stained nuclei (B, D, F, H). Arrows indicate the position of YSL nuclei. Magnification is indicated by the bar which indicates 100 μm.
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Reprinted from Developmental Biology, 285(2), Sharma, D., Holets, L., Zhang, X., and Kinsey, W.H., Role of Fyn kinase in signaling associated with epiboly during zebrafish development, 462-476, Copyright (2005) with permission from Elsevier. Full text @ Dev. Biol.