Fig. 6

Fig. 6  Cell fate analysis in the retinae of soxp knockdown animals. In panels A through H, counts were performed on frozen sections following the injection of an anti-soxp morpholino or a control morpholino. (A) Parvalbumin-positive GCL cells in the soxp knockdown and control retinae. Following morpholino treatment, the numbers of parvalbumin-positive GCL cells are reduced from 32 ± 8 cells per section to 9 ± 9 cells (mean ± SD; P < 0.001 t test). (B) HuC/HuD-positive amacrine cells. (C) Muller glia visualized with anti-carbonic anhydrase antibody. (D) Zpr1-positive red-green double cones. (E) NPY-positive amacrine cells. Following anti-soxp morpholino treatment, the numbers of NPY-positive amacrine cells are increased from to 1.3 ± 1.1 to 2.2 ± 1.2 per retinal section (mean ± SD; P < 0.05 Whitney–Mann test). (F) Tyrosine hydroxylase-positive interplexiform cells. (G) GABA-positive anacrine cells. (H) Serotonin-positive amacrine cells. (I, J) Antibody staining of parvalbumin-positive cells (green) and carbonic anhydrase-positive Müller glia (red) in soxp knockdown (J) and control knockdown retinae (I). (K, L) Staining of ganglion and amacrine cells with an anti-Hu antibody and of red-green cones with the Zpr-1 antibody (both blue) in control knockdown (K) and soxp knockdown (L) retinae. In both panels, sections are counterstained with phalloidin to visualize plexiform layers (green). (M, N) Staining of NPY-positive cells in soxp knockdown (N) and control knockdown retinae (M). Photoreceptor cell layer staining with the zpr-1 antibody is provided as a control (also in red). (O, P) Staining with anti-GABA antibodies in soxp knockdown (P) and control knockdown (O) retinae. Arrows in panels I and J indicate parvalbumin-positive cells in the ganglion cell layer. Arrowheads in panels M and N indicate NPY-positive cells in the INL. Asterisks indicate the optic nerve. C, control knockdown; L, lens; MO, soxp knockdown.