Fig. 5 Developmental expression pattern of zebrafish CTCF gene. Distribution of the CTCF message was analyzed at different developmental stages from 0.75 to 48 hpf (panels from A to H, respectively). At early stages, expression of CTCF is ubiquitous (A–E), becoming more restricted to the brain by late embryogenesis (F–H). Late embryos show CTCF transcript mostly in the gut (Fig. 6, panel H). As positive controls, evx1 transcript yielded a pattern similar to that reported at ZFIN (http://zfin.org; ZFIN ID: ZDB-XPAT-021126-6) (panel I). Negative control for in situ hybridization staining by sense CTCF exon 1 probe (panel J). Abbreviations: av, axial vessel; bar, brachial arches; fb, forebrain; hb, hindbrain; mb, midbrain; nch, notochord.
Reprinted from Gene, 375, Pugacheva, E.M., Kwon, Y.W., Hukriede, N.A., Pack, S., Flanagan, P.T., Ahn, J.C., Park, J.A., Choi, K.S., Kim, K.W., Loukinov, D., Dawid, I.B., and Lobanenkov, V.V., Cloning and characterization of zebrafish CTCF: Developmental expression patterns, regulation of the promoter region, and evolutionary aspects of gene organization, 26-36, Copyright (2006) with permission from Elsevier. Full text @ Gene