Fig. 9

Fig. 9  Cell death in the CNS of Stau2-depleted embryos. (A–D) TUNEL labeling showing dead cells in 20 somite stage embryos that were injected with control morpholino (A), GST (B, D), Stau2 MO2 (F), or Stau2 RBD5 (G, I). Immunostaining with an antibody towards acetylated tubulin to detect neurons (C, H) in embryos injected with GST (C–E) or Stau2 RBD5 peptide (H–J), and co-localization of acetylated tubulin with TUNEL positive cells (E, J). Lateral views, scale bar in panel B for panels A, B, F, G, 100 μm; panels C for panels C–E and H–J, 200 μm. (K) % embryos with TUNEL labeling at 12 hpf, 16 hpf, 19 hpf, and 24 hpf upon injection of control morpholino, stau2 morpholinos, GST peptide, or Stau2 RBD5 peptide.