Fig. 5

Fig. 5  Stau1 and Stau2 peptides overexpression abolishes PGCs. (A–F) Expression of the germ cell specific gene, vasa, in 4 cell (A, B), shield stage (C, D), and 24 hpf (E, F) embryos injected with GST (A, C, E), Stau2 RBD5 (B, D, F); GFP:nos1 expression in shield stage (G, H), 3-somite stage (I, J), and 24 hpf (K, L) embryos injected with GST (G, I, K), Stau2 RBD5 (H, J, L), histogram in M shows % embryos with GFP expression in PGCs. In panels I–L, dotted boxes outline the areas shown at higher magnification in the insets. A–D, animal pole views; E–H and K, L, lateral views; I, J dorsal views. Scale bars, 50 μm for panels A, C, G, I and 100 μm for panels E, K, and insets.