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Fig. S1 Zebrafish and sole bgp cDNAs. Nucleotide sequence of the cDNAs encoding zebrafish and sole Bgp. The bgp cDNAs were obtained by a combination of RT- and 5′ RACE-PCR amplification. Numbering on right side corresponds to nucleotides. Amino acid residues are numbered according to the first residue of the mature protein and are shown above (for zebrafish) or below (for sole) the corresponding codons in cDNA sequence. The polyadenylation signals are underlined twice and the stop codons are identified by their three letter code. Conserved cysteine residues are boxed. An asterisk is used to show identity between zebrafish and sole amino acid sequences. The sequences used for constructing primers are underlined and marked by horizontal arrows next to the name of the primer. Presumed Gla residues are shown in bold (based in homology with other bgp sequences). A 16 (AC) repetitive motif on zebrafish bgp 3′-UTR region is marked between curved arrows.
Reprinted from Gene expression patterns : GEP, 6(6), Gavaia, P.J., Simes, D.C., Ortiz-Delgado, J.B., Viegas, C.S., Pinto, J.P., Kelsh, R.N., Sarasquete, M.C., and Cancela, M.L., Osteocalcin and matrix Gla protein in zebrafish (Danio rerio) and Senegal sole (Solea senegalensis): Comparative gene and protein expression during larval development through adulthood, 637-652, Copyright (2006) with permission from Elsevier. Full text @ Gene Expr. Patterns