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Fig. 5 Morpholinos against TTN1 phenocopy the ruz mutation. (A) 3.5 dpf wild-type fish injected with the TTN1b morpholino against the translational start site of TTN1 (bottom) show decreased birefringence, mobility, and size and are slow to hatch compared with uninjected wild-type siblings (top). Similar results were seen upon injection with the non-overlapping TTN1a morpholino compared with inverted control-injected siblings (data not shown). (B) India ink staining of electrophoretically separated protein lysates of 3.5 dpf TTN1a morphants (MO) and uninjected siblings (uninj) shows loss of the two largest titin isoforms in TTN1 morphants. Smaller titin isoforms are thus likely coded by the adjacent TTN2 gene. (C, D) Longitudinal sections of zebrafish skeletal muscle at 3.5 dpf were stained with hematoxylin. (C) Wild-type muscle shows myofiber alignment between myosepta and peripherally localized nuclei. (D) TTN1a morphants have decreased myofiber alignment and abnormally shaped nuclei similar to ruz mutants.
Reprinted from Developmental Biology, 309(2), Steffen, L.S., Guyon, J.R., Vogel, E.D., Howell, M.H., Zhou, Y., Weber, G.J., Zon, L.I., and Kunkel, L.M., The zebrafish runzel muscular dystrophy is linked to the titin gene, 180-192, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.