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Fig. 1 Zebrafish lmo4b is essential for viability and eye, ear and pectoral fin development. (A) Alignment of amino acid sequence alignment of zebrafish (Dr.) Lmo4b (Accession #NP817093) with mouse (Mm.; Accession #NM010723.1), human (Hs.; Accession #NM006769.2) and zebrafish (Dr.) Lmo4a (Accession #AAN03596). Residues unique to Lmo4b are underlined in red. (B) Western blot of extracts from embryos injected with 500 pg of 6xmyc-lmo4b mRNA and a mixture of two non-overlapping translational-blocking MOs or a 4 mm control MO, probed with anti-myc antibody. An unrelated immunoreactive band present in all extracts demonstrates comparable loading for all lanes. (C–J) 72 hpf larvae or larval tissue from embryos injected with 4 ng of either 4 mm-MO (C, E, G, I) or 4 ng of lmo4b-MO (D, F, H, J). High magnification views of morphant eyes reveal retinal enlargement (F) and defects in the ear (G, H) and pectoral fins (I, J).
Reprinted from Developmental Biology, 309(2), McCollum, C.W., Amin, S.R., Pauerstein, P., and Lane, M.E., A zebrafish LMO4 ortholog limits the size of the forebrain and eyes through negative regulation of six3b and rx3, 373-385, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.