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Fig. S2 pbx2-MO; pbx4-MO embryos are rescued by exd. (A-D) RNA in situ expression of myog and krox-20 (blue) and myod (red). A and B are wild-type control siblings of C and D, which were injected with pbx2-MO; pbx4-MO. A and C were injected with gfp mRNA, and B and D were injected with exd mRNA. In this experiment, embryos were fixed at the same time, about 10s-stage for A,B,D and about 8s-stage for C. exd mRNA rescues krox-20 and myog expression in pbx2-MO; pbx4-MO embryos. exd mRNA also rescues the developmental delay (scored by myod expression) in pbx2-MO; pbx4-MO embryos. Embryos are shown in dorsal view, anterior towards the left. (E) Quantification of the rescue experiment by counting the number of somites expressing myog or myod. Error bars represent standard deviation.