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Fig. 7 Overexpression of scl and etsrp Promotes Vasculogenesis and Inhibits Heart Formation. In situ hybridization depicts expression of fli1a (A–C), hand2 (D–F), and cmlc2 (G–I) in wild-type (A, D, and G) and scl+etsrp RNA-injected embryos displaying severe (B, E, and H) and moderate (C, F, and I) phenotypes. Dorsal views, anterior to the top, at the 7 somite (A–F) and 30 hpf (G–I) stages. The scale bars represent 100 μm. (A–C) Overexpression of scl and etsrp induces ectopic expression of fli1a, including expression within the HFR (arrowheads; severe phenotype, n = 7/15; moderate phenotype, n = 5/15). (D–F) RNA-injected embryos exhibit reduced expression of hand2 (arrows; severe phenotype, n = 14/34; moderate phenotype, n = 15/34). (G–I) RNA-injected embryos exhibit small, dysmorphic hearts (severe phenotype, n = 9/11; moderate phenotype, n = 2/11).
Reprinted from Developmental Cell, 13(2), Schoenebeck, J.J., Keegan, B.R., and Yelon, D., Vessel and blood specification override cardiac potential in anterior mesoderm, 254-267, Copyright (2007) with permission from Elsevier. Full text @ Dev. Cell