ZFIN Image: Etard et al., 2007, Fig. 4

Image

Figure Caption

Fig. 4 Knockdown of Steif phenocopies the mutant phenotype. Two-day-old wild-type (A, B), morpholino-injected (C, D) and steif mutant embryo (E, F). Embryos (A, C, E) were illuminated at 72 hpf with polarized light to visualize the birefringence in the somitic muscle (for color representation see Fig. 3). Knockdown of Steif/Unc-45b protein (C) by injection of antisense morpholino reduces the birefringence similarly to mutations in steif (E). (B, D, F) wild-type (B), morpholino injected (D) and steif mutant embryos (F) stained immunohistochemically at 48 hpf with the antibody F59 directed against slow muscle myosin. Knockdown embryos (D) and steif (F) mutants have similarly disorganized slow muscle myofibrils. Scale bar: 50 μm (A, C, E); 15 μm (B, D, F).

Figure Data

Phenotype details

Acknowledgments

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Reprinted from Developmental Biology, 308(1), Etard, C., Behra, M., Fischer, N., Hutcheson, D., Geisler, R., and Strähle, U., The UCS factor Steif/Unc-45b interacts with the heat shock protein Hsp90a during myofibrillogenesis, 133-143, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.