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Fig. 5 Ubiquitous misexpression of Cxcl12a perturbs olfactory neuron assembly but does not affect projection of olfactory axons. omp:yfp/+ and omp:yfp/+;hsp:cxcl12a/+ zebrafish embryos were heat shocked at either 12 or 19 hpf. Using confocal microscope, positioning of olfactory neurons and subsequent projection of olfactory axons were viewed in the same individuals at 28 hpf (A-C) and 3.5 dpf (D-F), respectively. Composite images generated from a series of optical sections are shown. Heat induction of Cxcl12a at 12 hpf (B), but not 19 hpf (C), perturbs assembly of olfactory neurons. Heat shocking itself does not affect the positioning of olfactory neurons (A). None of the Cxcl12a heat-induced embryos display an impaired projection of olfactory axons at 3.5 dpf (D-E). All panels show frontal views. Scale bar: 100 µm. d, dorsal; v, ventral.