IMAGE

Fig. 4

ID
ZDB-IMAGE-070620-19
Source
Figures for Robu et al., 2007
Image
Figure Caption

Fig. 4 Representative Cell Death Phenotypes Detected at 26 hpf. Brightfield (A, B, D, E, G, H, J, and K) and darkfield images (C, F, I, and L) of 26 hpf zebrafish embryos injected with Wnt5 MO1. (M–X) TUNEL assay. Lateral views (A, D, G, J, M, N, P, Q, S, T, V, and W), with inserts showing a higher magnification of the head region (A, D, G, and J). Dorsal view of the head (B, C, E, F, H, I, K, L, O, R, U, and X). The two classes of phenotypes described in Figure 3 are observed at this time point, too (intermediate, G–I and P–R; severe, J–L and S–T; uninjected embryos, A–C and M–O). At 26 hpf, however, a milder cell death phenotype was also observed (D–F). This class of embryos exhibited an anterior-ventral concavity and a depressed hindbrain (indicated by the arrows), without tissue with obvious characteristic cell-death patterns featured by embryos with intermediate and severe phenotypes (compare [F] to [I] and [L]). However, even these mildly affected embryos showed clear apoptosis indicated by the TUNEL assay (P–R).

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.