ZFIN Image: Voas et al., 2007, Fig. 4

Image

Figure Caption

Fig. 4

Ultrastructural Analysis of the Node of Ranvier in αII-spectrin^st60 Mutants

(A–D) TEM images of the PLLn (A and B) and Mauthner axons of the ventral spinal cord (C and D) in transverse sections of 5 dpf larvae. Arrowheads indicate multiple layers of myelin present in both the wild-type (A and C) and αII-spn^st60 mutants ([B] and [D]; Ax = myelinated axon). For both the wild-type and mutants, the PLL nerves and spinal cords of at least six individuals were examined. Scale bars represent 0.5 μm.

(E and F) TEM images of nodes of Ranvier in longitudinal sections from the PLLn at 3 dpf. On average, the nodal gap between paranodes (indicated here with brackets) is slightly longer in αII-spn^st60 mutants (F) than in the wild-type at this stage (E).

(G and H) Nodes of Ranvier from the PLLn at 9 dpf. At this stage, the average nodal gap in αII-spn^st60 mutants (H) is significantly longer than that seen in wild-type nodes (G).

(I and J) Paranodes from longitudinal sections of the PLLn at 9 dpf. Morphology of the paranodal loops and transverse bands (indicated by arrowheads) are comparable between wild-type and mutant nodes. Scale bars for (E)–(J) represent 0.2 μm.

Figure Data

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Curr. Biol.