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Fig. S3 Cycloheximide inhibits translation. Transgenic flh:gfp (Gamse et al., 2003) embryos were treated with vehicle (DMSO) (A,C) or CHX (B,D) and scored for expression of Gfp by fluorescence. (A,B) Embryos treated at sphere stage and scored at 80% epiboly. Vehicle-treated control embryos (A) show normal levels of Gfp, while expression is barely detectable in embryos treated with CHX (B). (C,D) Embryos treated at 50% epiboly and scored at 100% epiboly. Gfp expression was much reduced in CHX-treated embryos compared to vehicle-treated controls. Note that transgenic Gfp expression had already begun by the start of the earliest CHX treatment at sphere stage. Gfp protein is very stable, making it likely that some of the remaining Gfp fluorescence is due to pretreatment synthesis.