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Fig. S2 Comparison of TOP:GFP and zic2aD5:gfp expression. Embryos were processed by ISH to detect gfp RNA. (A) Transgenics carrying the Wnt-responsive transgene TOP:GFP (Dorsky et al., 2002) show strong midbrain and weak hindbrain expression at 10 somites. (B) zic2aD5:gfp is weakly expressed in the midbrain and strongly expressed in the hindbrain at the same stage. (C,D) At a later stage (20 somites) TOP:GFP expression overlaps with zic2aD5:gfp, primarily in the midbrain. All views are lateral, anterior to the left. Arrowheads mark the tectal borders.
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