ZFIN Image: Curado et al., 2007, Fig. 1

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Figure Caption

Fig. 1 Tissue-specific Nitroreductase/Metronidazole (NTR/Mtz) cell ablation system. A: Mechanism of NTR/Mtz cell ablation: a cell expressing the bacterial NTR (NTR⁺; in cyan blue) when exposed to the prodrug Mtz (in green) converts the latter into a cytotoxic agent (in red), which causes DNA damage and death of the NTR⁺ cell. B,D: Constructs used to generate the Tg(cmlc2:CFP-NTR)^s890 and Tg(l-fabp:CFP-NTR)^s891 lines contain the cmlc2:CFP-NTR and l-fabp:CFP-NTR sequences flanked by tol2 sequences. C: The Tg(ins:CFP-NTR)^s892 sequence is flanked by I-Sce sites ("i"). E-G: Brightfield combined with fluorescence imaging of 4, 3, and 6 days postfertilization (dpf) larvae, respectively, showing stable expression of Cyan Fluorescent Protein-NTR (CFP-NTR; reported by CFP fluorescence) in cardiomyocytes (autofluorescence in the yolk, E), Insulin-producing pancreatic β-cells (F) and hepatocytes (G).

Acknowledgments

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