Fig. S1

Fig. S1 The αII-spectrin^st60 Node Defect Is Evident Soon after Sodium-Channel Clusters Form. PLLn nodes from wild-type and αII-spn^st60 embryos labeled with anti-panNa_vCh and anti-FIGQY (which labels nodal Neurofascin) at 2, 2.5, 3, and 4 dpf. (A′)–(H′) show anti-FIGQY alone, and (A″)–(H″) show anti-panNa_vCh alone. (A–B) At 2 dpf, the PLLn is diffusely labeled with both antibodies. The scale bar represents 5 μm. (C–D) At 2.5 dpf, sodium-channel and Neurofascin clusters are present and colocalize. The cluster morphology is immature, and there is no obvious difference between the wild-type (C) and mutant (D). (E–F) By 3 dpf, most of the wild-type sodium-channel and Neurofascin clusters have acquired mature node morphology (E). Many clusters in mutants have not achieved mature morphology (F). (G–H) At 4 dpf, sodium-channel and Neurofascin cluster morphology remains abnormal in the mutants (H).