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Figure Caption
Fig. 4 Mib is associated with Delta in Cos7 cells. (A, B) Western blot analysis of co-immunoprecipitation samples reveals the association between full-length Mib with DeltaD (upper panel) and HA-DeltaAICD (lower panel). (C) Schematic diagram of the Mib portion of EGFP fusion proteins. The solid vertical bars indicate the ankyrin repeats, the empty triangles indicate the ring fingers. (D, E) Co-IP analysis of Mib deletions and DeltaD shows that the N-terminal half is necessary for DeltaD association. (F) GST pull-down analysis shows that the N-terminal half is sufficient to associate with DeltaD.
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Reprinted from Developmental Biology, 267(2), Chen, W., and Casey Corliss, D., Three modules of zebrafish Mind bomb work cooperatively to promote Delta ubiquitination and endocytosis, 361-373, Copyright (2004) with permission from Elsevier. Full text @ Dev. Biol.