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Figure Caption
Fig. 4 Flow is not required for the development of AV conduction tissue. (A) Time line of the experiments with silent heart embryos. (B) Calcium imaging of silent heart embryo at 48 hpf. Compressed spacing of isochronal lines (50 mseconds) in the AV ring demonstrates physiological conduction delay. Scale bar: 50 μm. (C) silent heart mutant embryos develop 2:1 block at 48 hpf in response to terfenadine treatment, as demonstrated in this plot of atrial and ventricular fluorescence intensity.
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