Fig. 5

Fig. 5 Comparative expression of shh (A–D), ptc (E–H), and prechordal plate markers hgg1 and hlx1 (I–L) relative to dlx3, baseball-stitch band around the anterior edge of the neural plate in wild-type (A, E, I), kny^m119 (B, F, J), tri^m209 (C, G K), and kny^m119 tri^m209 (D, H, L) visualized by in situ hybridization. The shh expression domain in kny^m119 (B), tri^m209 (C), and kny^m119 tri^m209 (D) is localized abnormally with respect to the anterior edge of the neural plate at the one-somite stage. This abnormal localization is also observed for ptc1 expression. A gap is observed between ptc1 and dlx3 for tri^m209 (G) and kny^m119 tri^m209 (H) mutant embryos, but not for wild-type (E) or kny^m119 (F) embryos. In addition, a gap is observed between hlx1 and dlx3 for all embryos including wild type (I–L). hgg1 (red) is positioned anterior to the neural plate in wild-type (I), tri^m209 (K), and kny^m119 tri^m209 (L) embryos and underlies the neural plate in kny^m119 embryos (J). Arrows indicate the anterior extent of shh (A–D), ptc (E–H), or hlx1 (I–L) expression. Bar, 60 um.